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Lected and disrupted by sonication in lysis buffer (25 mM Tris, pH 7.8, 2 mM EDTA, 1 Triton X-100, and 10 glycerol). After centrifugation, aliquots of the supernatants were tested for luciferase activity using the luciferase assay system. Firefly luciferase activities were standardized for b-galactosidase activity.To detect the in vivo association of nuclear proteins with rat mmp-9 promoter, chr