Osidase for 24 h. The pGL-MMP-9-Luctransfected cells were pretreated with TG, KN-62, AG1296, SP600125, or TSIIA for 1 h and then incubated with LTA for 16 h. (C) Activation of wild-type (WT) and AP-1-mutated (mt-AP1) MMP-9 promoter constructs by LTA. Cells were cotransfected with respective promoter constructs for 24 h and then incubated with 50 g/ml LTA for 16 h. The values for beetle luciferase